Mature parental rainbow trout were taken from the rearing stocks of fish ponds the Yamagata Prefectural Fisheries Promotion Association, Inland Water Fisheries Center Station in Yuza-cho, Japan.Mature eggs collected from a single female were rinsed with isotonic physiological saline and were artificially inseminated with milt from a single male. After complete hardening of the chorion by water absorption, the embryos were placed in a small basket and incubated at an average temperature of 14.5}0.3 oC in a hatching box with a flow-system using well water.

O. mykiss embryo

Hatching

O. mykiss
Mature parental masu salmon were taken from the rearing stocks of fish ponds of Yamagata Prefectural Inland Water Fisheries Experiment Station in Yonezawa. Mature eggs collected from a single female were rinsed with isotonic physiological saline and were artificially inseminated with milt from a single male. After complete hardening of the chorion by water absorption, the embryos were placed in a small basket and incubated at an average temperature of 10.5}0.3 oC in a hatching box with a flow-system using well water.

O. masou embryo

Hatching

O. masou